Fig. 6
Collagen type I in forearm flexor digitorum muscles. a Representative images of muscle cross-sections immunostained for collagen type I. Scale bar in upper left panel = 50 μm and applies to each image. b Quantification of the collagen type I immunostaining, with percent area with collagen type I staining per mm2 shown. c Muscle lysates assayed using Western blot methods. M = denotes lanes loaded with a prestained protein ladder as a Marker. Left panel: Membrane strip with two lanes probed only with the 2nd antibody. Middle panel: Muscle lysate samples from two animals per group were loaded into lanes as shown. The membrane was probed with collagen type I antibody (using the same antibody as used for the immunohistochemistry in panel a). Right panel: Membrane strip with one lane loaded with purified rat tail collagen as a positive marker for collagen type I (~ 121 kDa). d The same membrane shown in Panel (d) stained with Ponceau red to show amount of protein loaded per lane. e Collagen type I levels in muscle lysates, quantified using ELISA. f Transforming growth factor β 1 (TGF-β1) levels in muscle lysates, quantified using ELISA. Symbols: *: p < 0.05, compared to TASK-Tx group; &: p < 0.05 and &&: p < 0.01, compared to a control group as shown. Mean ± 95% CI shown