Fig. 2

Images and quantification of CD68-immunopositive macrophages (stained red) in forearm tissues. Treated (TASK-Tx and C-Tx) animals received manual therapy to their upper extremities, bilaterally, while active control (TASK-Ac and C-Ac) animals received manual therapy to their lower extremities, bilaterally. a-d CD68+ cells in longitudinal sections of median nerves at the level of the wrist. e Numbers of CD68+ cells in median nerves at the level of the wrist. f-i CD68+ cells in flexor digitorum muscles, in cross-sections from mid-forearm regions. j Numbers of CD68+ cells per mm² in flexor digitorum muscles, in cross-sections from mid-forearm regions. k-n CD68+ cells in longitudinal sections of flexor digitorum tendons, in mid- to distal regions. o Numbers of CD68+ cells per mm² in flexor digitorum tendons, in mid- to distal regions. Abbreviations: ct = connective tissue, epi = epineurium in nerves and epitendon in tendons; N = median nerve; T-tendon. Scale bar in panel a = 100 μm and applies to panels b-d, k-n. Scale bar in panel e = 50 μm and applies to panels f-i. Symbols in e, j and o **: p < 0.01, compared to TASK-Tx group; ^&: p < 0.05 and ^&&: p < 0.01, compared to a control group as shown. Mean ± 95% CI shown