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Fig. 1 | BMC Musculoskeletal Disorders

Fig. 1

From: Cell spheroids are as effective as single cells suspensions in the treatment of critical-sized bone defects

Fig. 1

Study design. MSCs were isolated from human bone marrow aspirates from healthy donors, divided into 2 groups: (1) MSC spheroids and (2) MSC suspensions and osteogenically pre-differentiated for 7 days. For spheroid formation, the detached MSC suspension was resuspended in osteogenic medium and transferred into 96-well suspension plates, followed by an incubation of 2 days. MSC suspensions were osteogenically differentiated in culture flasks for another 3 days. Then, 5‧104 cells - either as suspension or as spheroid - were immobilized within fibrin gels and implanted into a 2 mm segmental bone defect in the right femora of 26 nu/nu NOD/SCID nude mice that was stabilized by an external fixator. Animals were observed for 6 weeks. Afterwards, μCT and histological analyses were performed

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