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Table 1 Primers used in qRT-PCRa analysis to assess osteoclast and osteoblast function

From: Anti-IL17 antibody Secukinumab therapy is associated with ossification in giant cell tumor of bone: a case report of pathologic similarities and therapeutic potential similar to Denosumab

Gene Name

Forward Primer

Reverse Primer

HPRT

TGAGGATTTGGAAAGGGTG

GAGGGCTACAATGTGATGG

TRAP

GACCACCTTGGCAATGTCTCTG

TGGCTGAGGAAGTCATCTGAGTTG

Cathepsin K

TGAGGCTTCTCTTGGTGTCCATAC

AAAGGGTGTCATTACTGCGGG

RANKL

GAGAAAGCGATGGTGGATGG

CTTGCTCCTCTTGGCCAGAT

MMP9

TGGGGGGCAACTCGGC

GGAATGATCTAAGCCCAG

OPG

AGAAAGGAAATGCAACACACGAC

CCTGAAGAATGCCTCCTCACAC

  1. a qRT-PCR reverse transcription-quantitative polymerase-chain-reaction
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BMC Musculoskeletal Disorders

ISSN: 1471-2474

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