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Fig. 2 | BMC Musculoskeletal Disorders

Fig. 2

From: The expression of substance P and calcitonin gene-related peptide is associated with the severity of tendon degeneration in lateral epicondylitis

Fig. 2

Quantitative image analyses of SP and CGRP expression. Quantitative analysis was achieved by counting the positive brown particles pixel-by-pixel in immunohistochemistry images using ImageJ (National Institute of Health, Bethesda, Maryland, USA). First, digital images of SP and CGRP immunostaining were obtained (a). Next, the color deconvolution plugin was used to isolate immunopositive particles into the brown image from the original image using the H-DAB vector (b). The positive particles of the brown image were converted into black and white images for recognition (c). Three montages of each image were generated for accurate measurements and threshold determination, and the positive signal area was measured in pixels (d). SP, substance P; CGRP, calcitonin gene-related peptide; magnification, × 400; scale bar = 50 μm

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