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Table 2 The application of CRISPR/Cas9 in Ewing’s sarcoma research

From: Applications of CRISPR/Cas9 in the research of malignant musculoskeletal tumors

Target Genes Cell lines CRISPR/Cas9 Applications Effects References
EWSR1,FLI1 hMSC,hiPSC,HEK293A,HEK293T induce chromosomal translocation EWSR1-FLI1 chromosomal translocation cell model [69,70,71]
MDM2,MDM4,TP53,USP7,PPM1D TC32,TC138
A673,EWS502
knock out Proliferation↓,
chemical cytotoxity↓
[72]
PHF19 SK-N-MC knock out Proliferation↓,colony formation↓, invasion↓, JQ1 sensitivity↑ [73]
TNC A673,SKNMC knock out Proliferation↓,migration↓,
metastases in mice↓,
Hippo/YAP pathwayactivity↓,MALAT1,
P-SRC,P-MYC expression↓
[74]
MSH2 A673 knock out microsatellite instability (MSI)↑,
somatic mutation frequencies ↑,
compound-resistant to cellular toxins: PSMB5,CD437,MLN4924↑
[75]
  1. Notes: ↑:Up-regulation: ↓:Down-regulation