Table 2 The application of CRISPR/Cas9 in Ewing’s sarcoma research

EWSR1,FLI1

hMSC,hiPSC,HEK293A,HEK293T

induce chromosomal translocation

EWSR1-FLI1 chromosomal translocation cell model

[69,70,71]

MDM2,MDM4,TP53,USP7,PPM1D

TC32,TC138

A673,EWS502

knock out

Proliferation↓,

chemical cytotoxity↓

[72]

PHF19

SK-N-MC

knock out

Proliferation↓,colony formation↓, invasion↓, JQ1 sensitivity↑

[73]

TNC

A673,SKNMC

knock out

Proliferation↓,migration↓,

metastases in mice↓,

Hippo/YAP pathwayactivity↓,MALAT1,

P-SRC,P-MYC expression↓

[74]

MSH2

A673

knock out

microsatellite instability (MSI)↑,

somatic mutation frequencies ↑,

compound-resistant to cellular toxins: PSMB5,CD437,MLN4924↑

[75]