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Fig. 1 | BMC Musculoskeletal Disorders

Fig. 1

From: The inhibition by human MSCs-derived miRNA-124a overexpression exosomes in the proliferation and migration of rheumatoid arthritis-related fibroblast-like synoviocyte cell

Fig. 1

The preparation and characterization of HMSC-124a-EV. a The specificity biomarker of human MSC was detected by Flow cytometry; b TEM imaging of prepared exosomes; c The size distribution of exosome evaluated by Dynamic light scattering; d Zeta potential of the prepared HMSC-124a-EV; e The qualitative analysis of CD9 and CD63 within exosomes by western blot; f The detection of CD9 and CD63 by Flow cytometry; g Relative miRNA-124a level in HMSC-124a-EV measured by RT-PCR; h Fluorescent imaging of MH7A cells after the co-incubation with HMSC-124a-EV, blue represents nucleus and red represents the exosome phagocytosed by MH7A cell; i Relative miRNA-124a level in MH7A cells after the incubation with HMSC-124a-EV. n = 5, ***p < 0.001 as determined by Student’s t-test. All these data were representative of two independent experiments

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BMC Musculoskeletal Disorders

ISSN: 1471-2474

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