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Table 1 Primers used for RT-PCR

From: Different effects of Wnt/β-catenin activation and PTH activation in adult and aged male mice metaphyseal fracture healing

Genes

Primer Forward Sequence(5′, 3′)

Primer Reverse Sequence

β-catenin

ACGGTGCCGCGCCGCTTATA

TAGCCATTGTCCACGCAGCG

RUNX2

AAGTGCGGTGCAAACTTTCT

TCTCGGTGGCTGGTAGTGAG

PTH1R

AGCGAGTGCCTCAAGTTCAT

ACAGCGTCCTTCACGAAGAT

GAPDH

GAGAAGGCTGGGGCTCATTT

CCAATATGATTCCACCCATG

OCN

AGGGCAGCGAGGTAGTGA

TGGTGTAGCCGAAAGTCC

VEGF

CGATGAAGCCCTGGAGTG

ATGATGGCGTGGTGGTGA

  1. Shown are the details of the primers used for RT-PCR, including the forward (F) and reverse (R) sequences. RUNX2 Runt-related transcription factor 2, PTH1R Parathyroid hormone 1 receptor, GAPDH Glyceraldehyde-3-phosphate dehydrogenase, OCN Osteocalcin, VEGF Vascular endothelial growth factor
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BMC Musculoskeletal Disorders

ISSN: 1471-2474

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