Fig. 5

RT-PCR expression levels of a β-catenin, b PTH1R, c RUNX2, d OCN, and e VEGF RT-PCR expression levels and immunostaining for f β-catenin, g PTH1R, and h BrdU at the metaphyseal defect at day 14 after fracture in the aged wild-type (WT-aged) mice, aged Wnt/β-catenin activation (CA-aged) mice, and aged PTH activation (PTH-aged) mice. The mRNA expression levels on the day of fracture were used as an internal control. n = 4 per group. i-k Quantification of the numbers of positive cells was performed. ^*P < 0.05 and ^**P < 0.01