Fig. 3

a β-catenin, b PTH1R, c RUNX2, d OCN, and e VEGF RT-PCR expression levels. The mRNA expression levels on the fracture day were used as an internal control. Immunostaining for (f) β-catenin, g PTH1R, and h BrdU at the metaphyseal defect at day 14 after fracture in adult wild-type (WT-adult) mice, adult Wnt/β-catenin activation (CA-adult) mice, and adult PTH activation (PTH-adult) mice. i-k Quantification of the number of positive cells was performed. n = 4 per group. ^*P < 0.05 and ^**P < 0.01