Table 1 Primer sequences and product sizes for real time RT-PCR
Gene | RT-PCR primer sequences (5′-3′) | Annealing temp. (°C) | Product size (bp) |
|---|---|---|---|
Chondrogenic marker genes | |||
 COL2A1 | Sense: TTTCCCAGGTCAAGATGGTC Antisense: CTTCAGCACCTGTC CACCA | 58 | 374 |
 SOX9 | Sense: AGTACCCGCACTTGCACAAC Antisense: CGTTCTTCACCGACTTCCTC | 58 | 263 |
 ACAN | Sense: TCGAGGACAGCGAGGCC Antisense: TCGAGGGTGTAGCGTGTAGAGA | 54 | 392 |
Hypertrophy and osteogenic marker genes | |||
 COL10A1 | Sense: CCCAACACCAAGACACAGTTC Antisense: GACTTCCGTAGCCTGGTTTTC | 54 | 468 |
 ALPL | Sense: GGAACTCCTGACCCTTGACC Antisense: CCACCATCTCGGAGAGTGAC | 51 | 454 |
Internal control | |||
 EEF1A1 | Sense: TGCCCCTCCAGGATGTCTAC Antisense: CACGGCCCACAGGTACTG | 60 | 59 |