Skip to main content
Fig. 7 | BMC Musculoskeletal Disorders

Fig. 7

From: Reduced hypertrophy in vitro after chondrogenic differentiation of adult human mesenchymal stem cells following adenoviral SOX9 gene delivery

Fig. 7

Temporal gene expression profiles determined by real time RT-PCR in MSC pellet cultures genetically modified with SOX9 compared to TGFB1 or IGF1 alone or in combination. Profiles of temporal gene expression determined by real time RT-PCR in MSC pellet cultures after chondrogenic induction using adenoviral vectors encoding SOX9, SOX9 + TGFB1, TGFB1, SOX9 + IGF1 or IGF1. Genes analysed include collagen (COL) type II (COL2A1), SOX9, aggrecan (ACAN), COL type X (COL10A1), alkaline phosphatase (ALPL). Primer sequences and product size are listed in Table 1, with elongation factor 1α (EEF1A1) serving as housekeeping gene and internal control. For each marrow preparation/patient, treatment group and time point RNA was extracted from 10 pellets. Values of real-time RT-PCR analyses represent mean expression ratios ± SD normalised to the expression levels of the housekeeping gene EEF1A1 and the GFP controls as determined by the ΔΔCT method, with significant differences (p < 0.05) relative to values from the non-chondrogenic IGF1 group being marked with asterisks (*).

Back to article page