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Fig. 5 | BMC Musculoskeletal Disorders

Fig. 5

From: Reduced hypertrophy in vitro after chondrogenic differentiation of adult human mesenchymal stem cells following adenoviral SOX9 gene delivery

Fig. 5

Analysis for cell viability and apoptosis within MSC pellets after chondrogenic induction with adenoviral SOX9, TGFB1 or IGF1 gene transfer alone or in combination. Following genetic modification with Ad.GFP (Controls; a) Ad.SOX9 (b), Ad.SOX9 + Ad.TGFB1 (c), Ad.TGFB1 (d), Ad.SOX9 + Ad.IGF1 (e) or Ad.IGF1 (f) and at 5 × 102 vp/cell and aggregate culture. Pellets were double-stained with CFDA (left panels) and annexin 5 (right panels) at day 10 and 21 of culture. Fluorescence microscopy images of representative sections are shown. Note that living cells are stained green with CFDA, late apoptotic cells red with annexin 5-Cy3, while early apoptotic cells stained for both CFDA and Annexin 5. Panels are reproduced at low (50x; bar = 200 μm) or high (200x; bar = 50 μm) magnification as indicated

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BMC Musculoskeletal Disorders

ISSN: 1471-2474

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