Fig. 6

Flexor digitorum muscle pathology. a and b Representative H&E stained images of flexor digitorum muscles show an absence of enlarged immune cells or intramuscular fibrosis in FRC and TR + TM animals. c Representative H&E stained images of a 10-wk HRHF rat muscle showing the presence of immune cells (indicated by arrows). Inset shows additional examples of immune cells (arrows). d Representative H&E stained image showing the presence of intramuscular fibrosis (f ct) in a 10-wk HRHF+TM rat muscle. e and f Arrows indicate examples of CD68+ macrophages (stained black) in muscles of 10-wk HRHF and 10-wk HRHF+TM rats (eosin counterstaining). Scale bar = 50 μm; ct = loose areolar connective tissue; f ct = fibrotic, aka thickened, connective tissue; E = eosin, M = muscle)