Fig. 7

Effect of polyphyllin VII on components of the TRAF6/c-Src/PI3K signal transduction pathway. BMMs cells were pretreated with various concentrations of polyphyllin VII (PP7). The cells were the induced to differentiate into osteoclasts with or without RANKL and M-CSF. After 48 h, protein levels of the molecules in RAF6/c-Src/PI3K signaling pathway were assessed with immunoblotting. Panel a Representative image of immunoblotting of TRAF6, c-Src, p-PI3k and PI3k protein. Panel b, c, d and e protein expression level of TRAF-6 (b), c-Src (c), p-PI3K (d), and PI3K (e). Vertical axes: protein expression level; horizontal axes: polyphyllin VII (PP7) concentrations (μM). * P < 0.05 as compared to the cells treated with 0 μM of polyphyllin VII