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Fig. 2 | BMC Musculoskeletal Disorders

Fig. 2

From: Polyphyllin VII attenuated RANKL-induced osteoclast differentiation via inhibiting of TRAF6/c-Src/PI3K pathway and ROS production

Fig. 2

Inhibitory effect of polyphyllin VII (PP7) on the osteoclast differentiation from BMMs. BMMs were induced to differentiate into osteoclasts with or without RANKL and M-CSF in the presence of various concentrations of polyphyllin VII (PP7). The osteoclasts were visualized by TRAP staining. Panel a BMMs cells stained with TRAP. Cells with multiple red nuclei indicated osteoclasts. Magnification: × 100. Panel b Number of TRAP positive cells in each well. Vertical axis: osteoclast cell number; horizontal axis: polyphyllin VII (PP7) concentration (μM). Panel c Relative activity of TRAP in polyphyllin VII-treated cells compared to the control cell. Vertical axis: TRAP-5B level; horizontal axis: polyphyllin VII (PP7) concentration (μM). * P < 0.05, compared to the cells treated with 0 μM of polyphyllin VII (PP7); the dotted line represents a regression line

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