Table 1 The sequences of primers of RT-qPCR
From: Elevated lymphotoxin-α (TNFβ) is associated with intervertebral disc degeneration
Gene Name | Forward/ Reverse | 5′-3′Sequence | Size |
|---|---|---|---|
LTα | Forward | CCTGGCTGCACTCGATGT | 127 bp |
Reverse | GCGAAGGCTCCAAAGAAG | ||
Caspase-3 | Forward | CTGGACTGCGGTATTGAG | 102 bp |
Reverse | GGGTGCGGTAGAGTAAGC | ||
Caspase-1 | Forward | CAGGAGGGAATATGTGGG | 120 bp |
Reverse | AACCTTGGGCTTGTCTTT | ||
MMP-3 | Forward | ACCTATTCCTGGTTGCTG | 105 bp |
Reverse | GGTCTGTGGAGGACTTGTA | ||
Aggrecan | Forward | TGAAACCACCTCTGCATTCCA | 96 bp |
Reverse | GACGCCTCGCCTTCTTGAA | ||
Type II collagen | Forward | GTCACAGAAGACCTCACGCCTC | 81 bp |
Reverse | TCCACACCGAATTCCTGCTC | ||
GAPDH | Forward | TCAAGAAGGTGGTGAAGCAGG | 115 bp |
Reverse | TCAAAGGTGGAGGAGTGGGT |