Fig. 4
From: Elevated lymphotoxin-α (TNFβ) is associated with intervertebral disc degeneration
Effects of LTα on degeneration-related molecules in rat NP cells. (A) P3 NP cells were plated on 6-well culture plates. LTα (5 μg/ml) and an equal volume of PBS were added to the experimental and control groups for 72 h, respectively. Total proteins were extracted for the WB analysis. Full image WB images have been provided in Supplementary 1. (B) Quantitative analysis of protein levels as determined in “A”. (C) Treatment conditions were the same as that in “A”. Total mRNA was extracted for RT-qPCR analysis. *, p < 0.05; **. p < 0.01; ***. p < 0.001 (One-way ANOVA, Tukey’s multiple comparison analysis)