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Fig. 1 | BMC Musculoskeletal Disorders

Fig. 1

From: Elevated lymphotoxin-α (TNFβ) is associated with intervertebral disc degeneration

Fig. 1

Images of intervertebral disc degeneration and detection of LTα in human NP tissues. (A) Upper panel: normal disc in a MRI T2 image; bottom panel: degenerated disc in a MRI T2 image. (B) Protein lysates from NP from the control and degeneration groups were blotted with anti-LTα and anti-GAPDH antibodies. (C) Quantitative analysis of LTα protein levels as determined in “B”. (D) Concentration of LTα in NP tissue determined by ELISA. (E) LTα mRNA production in NP tissue determined by RT-qPCR. Control group: relatively healthy disc samples from patients with idiopathic scoliosis or spinal trauma; degeneration group: degenerated disc samples from patients with lumbar spinal stenosis or discogenic low back pain. The GAPDH housekeeping gene/protein was used as the internal control. **. p < 0.01; ***. p < 0.001 (Unpaired, two-tailed Student’s t-test)

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