Fig. 3

Dysferlin and myogenin expression in dysf mut/mut muscle cells. a Representative WB of myogenin and dysferlin with oprozomib and EB1089 treatment. C+: untreated wild-type myotubes, (C-) untreated dysf mut/mut myotubes. Desmin was used as loading control. b Quantification of WB bands of dysferlin or myogenin expression. The highest expression of dysferlin and myogenin was reached using oprozomib at 10 nM. c Representative WB of myogenin and dysferlin with ixazomib and EB1089. d Quantification of WB showed that 100 nM of ixazomib presented the highest expression of dysferlin and myogenin. Data are represented as the mean of 3 replicates ± standard deviation. The fold-change is calculated over the results of dysferlin expression in WT non-treated myotubes. Results were statistically analyzed using one-way Anova followed by Tukey post hoc test. Statistical significance was set at p < 0.05