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Fig. 2 | BMC Musculoskeletal Disorders

Fig. 2

From: Proteasome inhibitors reduce thrombospondin-1 release in human dysferlin-deficient myotubes

Fig. 2

Proteasome inhibition profile. The three distinct ATPase-independent proteolytic activities (CT-L, C-L and T-L) of the proteasome were assayed using a chemioluminiscence-based method. Dysf mut/mut myotubes were pretreated with oprozomib and EB1089 for 24 h. Oprozomib treatment presented inhibition at: a CT-L at all concentrations tested b) C-L, and c) T-L activity also were inhibited by oprozomib, but only at high doses (50 nM and 100 nM). Pre-treatment with ixazomib and EB1089 for 8 h was also assayed and d) CT-L, e) C-L and f) T-L activity were inhibited at all doses tested with significant differences. Data are represented as mean of 3 replicates ± standard deviation. Results were statistically analyzed using one-way Anova followed by Tukey post hoc test. Statistical significance was set at p < 0.05. *p < 0.05; **p < 0.01; ***p < 0.0001

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