Skip to main content

Table 2 Primer sequences used for the Real-Time Polymerase Chain Reaction

From: Correlation between the HIF-1α/Notch signaling pathway and Modic changes in nucleus pulposus cells isolated from patients with low back pain

Gene   Primer sequence
Notch 1 Forward F5’-GCGACAACGCCTACCTCTG
Reverse R5’-AAGCCATTGATGCCGTCC
Notch 2 Forward F5’-TCAGCCGGGATACCTATGAG
Reverse R5’-CTGGCAGTGTCCTGGAATGT
Notch 3 Forward F5’-ACCGCGTGGCTTCTTTCTA
Reverse R5’-GAGCACTCGTCCACATCCTG
Notch 4 Forward F5’-GGAGAAGGGGCTGTGGAAT
Reverse R5’-GCAGGGGTCAGGAAACTGG
HES 1 Forward F5’-GAGTGCATGAACGAGGTGAC
Reverse R5’-GGTCATGGCATTGATCTGG
HIF1α Forward F5’-ACCACCTATGACCTGCTTGG
Reverse R5’-TATCCAGGCTGTGTCGACTG
GAPDH Forward F5’-GCACCGTCAAGGCTGAGAAC
Reverse R5’-TGGTGAAGACGCCAGTGGA