Table 2 Primer sequences used for the Real-Time Polymerase Chain Reaction
Gene | Primer sequence | |
|---|---|---|
Notch 1 | Forward | F5’-GCGACAACGCCTACCTCTG |
Reverse | R5’-AAGCCATTGATGCCGTCC | |
Notch 2 | Forward | F5’-TCAGCCGGGATACCTATGAG |
Reverse | R5’-CTGGCAGTGTCCTGGAATGT | |
Notch 3 | Forward | F5’-ACCGCGTGGCTTCTTTCTA |
Reverse | R5’-GAGCACTCGTCCACATCCTG | |
Notch 4 | Forward | F5’-GGAGAAGGGGCTGTGGAAT |
Reverse | R5’-GCAGGGGTCAGGAAACTGG | |
HES 1 | Forward | F5’-GAGTGCATGAACGAGGTGAC |
Reverse | R5’-GGTCATGGCATTGATCTGG | |
HIF1α | Forward | F5’-ACCACCTATGACCTGCTTGG |
Reverse | R5’-TATCCAGGCTGTGTCGACTG | |
GAPDH | Forward | F5’-GCACCGTCAAGGCTGAGAAC |
Reverse | R5’-TGGTGAAGACGCCAGTGGA |