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Fig. 3 | BMC Musculoskeletal Disorders

Fig. 3

From: LncRNA SNHG9 is downregulated in osteoarthritis and inhibits chondrocyte apoptosis by downregulating miR-34a through methylation

Fig. 3

Overexpression of SNHG9 led to the downregulation of miR-34a through methylation in chondrocytes from OA patients. In order to analyze the potential interaction between SNHG9 and miR-34a, chondrocytes from OA patients and healthy controls were transfected with SNHG9 expression vector or miR-34a mimic. Overexpression of SNHG9 and miR-34a in two types of chondrocyte was confirmed at 48 h post-transfection by qPCR (a). The effects of overexpression of SNHG9 on miR-34a (b) and the effects of overexpression of miR-34a on SNHG9 (c) in both types of chondrocyte were analyzed by qPCR. MSP was performed to analyze the effects of overexpression of SNHG9 on the methylation of miR-34a gene in both types of chondrocyte (d). All experiments were performed in triplicate manner and mean values were calculated and presented. *, p < 0.05

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BMC Musculoskeletal Disorders

ISSN: 1471-2474

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