Fig. 5

Effect of miR-410-3p on proinflammatory cytokines and the NF-κB pathway in vivo. a, qRT-PCR detection of transcript levels of miR-410-3p in articular cartilage samples from Sham-operated mice, OA mice infected with control lentivirus (OA + LV-NC), and OA mice infected with miR-410-3p-expressing lentivirus (OA + LV-miR-410-3p mimics). b, ELISA detecting the production of IL-1β, (c), IL-6 and (d), TNF-α on synovial fluid in Sham-operated, OA + LV-NC and OA + LV-miR-410-3p mimics mice. e, Western blot analysis showing representative blotting band intensities of detecting protein expression of HMGB1, IkBα, and p65 in chondrocytes of Sham-operated, OA + LV-NC and OA + LV-miR-410-3p mimics mice. f, Quantification data of western blot analysis showing the expression of HMGB1, (g), IkBα, and (h), p65 in Sham-operated, OA + LV-NC and OA + LV-miR-410-3p mimics mice. β-actin was used as a loading control. The value of the control was set at ‘1’ in qRT-PCR analysis. Each experimental group included 6 mice (n = 6). *P < 0.05, **P < 0.01 vs Sham-operated; #P < 0.05, ##P < 0.01 vs OA + LV-NC