Fig. 4

MiR-410-3p regulates the NF-κB pathway via HMGB1. a, Effect of overexpression of miR-410-3p on LPS-induced NF-κB activity in mouse primary chondrocytes. b, Western blot analysis detecting protein expression of HMGB1 in control, miR-410-3p mimics + empty vector, and miR-410-3p mimics + HMGB1 vector mouse primary chondrocytes. c, Effect of miR-410-3p on NF-κB activity after restoring HMGB1 expression. d, Annexin V-PI staining assay detecting cell apoptosis after restoring HMGB1 expression. **P < 0.01 vs Control or NC; #P < 0.05, ##P < 0.01 vs LPS + NC or miR-410-3p mimics + vector