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Fig. 2 | BMC Musculoskeletal Disorders

Fig. 2

From: MicroRNA-410-3p modulates chondrocyte apoptosis and inflammation by targeting high mobility group box 1 (HMGB1) in an osteoarthritis mouse model

Fig. 2

Differential expression of miR-410-3p in OA mice and the effect of overexpressing miR-410-3p in LPS-induced injury in mouse primary chondrocytes. a, qRT-PCR detection of transcript levels of miR-410-3p in articular cartilage tissues in OA mice, Sham-operated mice and control mice. b, qRT-PCR detection of transcript levels of miR-410-3p in LPS-treated and control chondrocytes. c, MTT assay detecting chondrocyte proliferation without LPS treatment. d, MTT assay detecting chondrocyte proliferation after LPS treatment and overexpression of miR-410-3p. e, Annexin V-PI staining assay detecting cell apoptosis in control, LPS treated chondrocyte (LPS + NC), and overexpression of miR-410-3p in LPS treated chondrocyte (LPS + miR-410-3p mimics). f, ELISA detecting the production of IL-1β, (g), IL-6 and (h), TNF-α in control, LPS + NC and LPS + miR-410-3p mimics on the supernatant of mouse primary chondrocytes. Each experimental group included 6 mice (n = 6). The value of the control was set at ‘1’ in qRT-PCR analysis. **P < 0.01 vs Control or Sham-operated; #P < 0.05, ##P < 0.01 vs LPS + NC

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