Fig. 4
Photomicrographs showing resveratrol prevents aged-dependent histological changes in rat femur. Upper left panel: the shaft of femur of a control rat, showing the outer periosteum formed of an outer fibrous layer (f) and an inner osteogenic layer (O) which contains osteoprogenitor (Op) and osteoblast (Ob) cells and osteocytes in their lacunae (arrow). Subperiosteal grooves (G) are also seen. Upper middle panel: the shaft of aged rat, showing the outer periosteum formed of an outer fibrous layer (f) and an inner osteogenic layer (O) which contains osteoprogenitor (Op) and osteoblast (Ob) cells. Subperiosteal grooves (G) are also seen. Refractile area is seen (*). Notice that the periosteum appears thinner as compared to the control. Upper right panel: the shaft of the resveratrol treated aged rats showing the outer periosteum formed of an outer fibrous layer (f) and an inner osteogenic layer (O) which contain osteoprogenitor (Op) and osteoblast (Ob) cells and osteocytes in their lacunae (arrow). Notice that the periosteum appears thicker as compared to the aged, and nearly similar to the control rats. Middle left panel: the shaft of a control rat, showing wide Haversion system (H) surrounded by osteocytes in their lacunae. Middle central panel: the shaft of an aged rat, showing narrow Haversion system (H) surrounded by osteocytes in their lacunae and some lacunae are empty (arrow). Middle right panel: the shaft of a resveratrol treated aged rat, showing wider Haversion system (H) than aged and nearly similar to control surrounded by osteocytes in their lacunae. Lower left panel: the diaphysis of control rat showing multinucleated acidophilic osteoclast in Howships lacunae with ruffled border (arrow) lower middle panel: the shaft of an aged rat, showing multinucleated acidophilic osteoclast in Howships lacunae (arrow) surrounded by areas of erosion and osteolytic area of faintly stained bone. Lower right panel: the diaphysis in resveratrol treated aged rat, showing multinucleated acidophilic osteoclast in Howships lacunae with ruffled border (arrow). (H&E 1000X)