Effects of short- and long-term glucocorticoid-induced osteoporosis on plasma metabolome and lipidome of ovariectomized sheep

Table 4 Effect of OVX and OVXG on the lipidome of sheep in the long-term analysis

m/z	Lipid class	OVX vs Control						OVXG5 vs OVX					OVXG2 vs OVXG5				p
		FC	FC	FC	FC	FC	FC	FC	FC	FC	FC	FC	FC	FC	FC	FC
		1	2	3	4	5	1	2	3	4	5	1	2	3	4	5	Trt	Time	Int
725.492	CL 72:6	1.20	0.91	1.16	0.98	1.05	0.89	0.96	0.99	1.06	0.90	1.06	1.05	0.91	0.90	1.04	0.018	0.082	0.023
752.511	CL 76:7	1.15	0.89	1.21	0.87	1.11	0.95	0.82	0.94	1.15	0.75	1.10	1.09	0.82	0.94	1.17	0.088	0.023	0.016
726.496	CL 72:5	1.19	0.91	1.19	0.95	1.02	0.88	0.98	0.87	1.06	0.88	1.19	1.05	1.01	0.97	1.05	0.03	0.026	0.018
751.518	CL 76:8	1.23	0.92	1.26	1.02	1.12	0.88	0.98	0.92	1.07	0.84	1.27	0.87	0.90	0.86	1.03	0.011	0.06	0.042
800.511	CL 84:15	1.15	0.85	1.16	0.97	1.07	0.92	0.93	0.87	0.99	0.88	1.03	1.26	1.10	0.93	1.04	0.027	0.06	0.005
686.546	CerP 39:1	1.21	0.91	1.21	1.02	1.09	0.97	0.93	0.99	1.08	0.80	1.08	1.22	0.86	0.83	1.13	0.042	0.071	0.014
660.543	CerP 37:0	1.17	0.93	1.16	0.99	1.10	0.94	0.91	0.88	1.04	0.92	1.04	1.13	0.91	0.99	1.00	0.019	0.029	0.045
785.429	PI 31:5	1.31	0.87	1.42	0.90	1.09	0.85	0.85	0.93	1.11	0.77	1.24	1.13	0.82	0.89	1.18	0.022	0.022	0.016
557.236	PI 14:0	1.43	0.73	1.52	0.81	1.14	0.89	0.76	1.02	1.20	0.80	1.20	1.30	0.69	0.83	1.03	0.12	0.153	0.019
543.316	PA 25:3	1.23	0.81	1.33	0.92	1.06	0.92	0.89	0.91	1.09	0.94	1.19	1.25	0.88	0.83	0.99	0.117	0.103	0.018
489.253	PA 21:2	1.40	0.79	1.31	0.94	0.93	0.86	0.94	0.90	1.24	0.90	1.13	1.13	0.86	0.79	1.04	0.099	0.046	0.024
464.323	lysoPE 18:0	1.44	0.83	1.41	0.93	1.19	0.77	0.88	0.82	1.04	0.92	1.33	1.16	0.95	0.90	0.93	0.014	0.004	0.043
558.243	PS 20:4	1.36	0.85	1.32	1.02	0.93	0.90	0.83	0.91	1.22	0.88	1.12	1.20	0.86	0.80	1.09	0.073	0.036	0.038
861.656	PG 42:0	1.39	0.84	1.22	1.00	0.89	0.89	0.93	0.90	1.26	0.98	1.03	1.16	0.92	0.80	0.99	0.138	0.185	0.026

Relative intensities were measured in plasma samples of control group (n = 5), OVX group (n = 6), OVXG2 (n = 3) and OVXG5 (n = 3) using a linear mixed model. Results are fold changes (FC) of the lipid abundance between the OVXG5 and the OVX groups and between the OVXG2 and the OVXG5 groups from month one to month five, among identified compounds by LC-MS. FC with a value > 1 indicated a relatively higher intensity present in treated animals, whereas a value < 1 indicated a relatively lower intensity compared with their respective control animals
All FC values, which are in bold, are significant at p < 0.05 calculated using post-hoc Fisher’s Least Significant Difference (LSD) test for pair-wise multiple comparison of the group means
CL cardiolipin, CerP ceramide 1-phosphates, PI phosphatidylinositol, PA phosphatidic acid, lysoPE lysophosphatidylethanolamines, PS phosphatidylserines, PG phosphatidylglycerol

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