Effects of short- and long-term glucocorticoid-induced osteoporosis on plasma metabolome and lipidome of ovariectomized sheep

Table 2 Effect of OVX and OVXG on the metabolite profile of sheep in the long-term analysis

m/z	Metabolite	OVX vs Control						OVXG5 vs OVX					OVXG2 vs OVXG5				p
		FC	FC	FC	FC	FC	FC	FC	FC	FC	FC	FC	FC	FC	FC	FC
		1	2	3	4	5	1	2	3	4	5	1	2	3	4	5	Trt	Time	Int
233.803	5-Methoxytryptophan	2.11	3.33	2.50	2.83	2.24	1.39	1.09	1.08	0.92	1.06	1.31	0.80	0.40	0.79	0.45	0.001	< 0.0001	0.015
148.039	Methionine	0.97	0.79	0.81	0.92	1.98	1.18	3.42	1.10	0.98	1.46	1.58	1.08	1.53	1.67	0.36	0.098	0.003	0.012
161.903	5-Hydroxylysine	0.68	1.14	0.96	0.93	1.06	1.74	1.10	0.67	1.19	0.83	1.18	1.09	1.67	1.07	1.15	0.335	0.374	0.007
164.071	L-Phenylalanine	0.59	1.13	0.78	1.18	1.13	2.22	1.19	0.70	1.09	0.96	1.28	1.09	1.98	1.11	1.03	0.057	0.229	0.003
144.044	Indole-3-carboxaldehyde	0.57	1.06	0.97	1.27	1.52	3.18	1.41	0.65	0.87	1.03	0.96	1.40	1.39	0.83	0.44	0.493	< 0.0001	< 0.0001
116.070	L-Valine	0.70	1.05	0.83	1.16	1.15	1.95	1.07	0.74	0.82	1.01	1.00	1.26	1.59	1.29	0.79	0.418	0.136	0.011
133.043	Malic acid	0.91	1.21	1.17	0.93	1.52	1.10	1.02	1.03	0.82	1.03	1.09	0.98	0.56	1.26	0.95	0.643	0.027	0.03
203.138	D-Tryptophan	0.57	1.07	0.70	1.05	0.99	1.93	1.24	0.83	0.79	0.75	1.35	0.82	1.25	0.90	1.07	0.673	< 0.0001	0.001

Relative intensities were measured in plasma samples of control group (n = 5), OVX group (n = 6), OVXG2 (n = 3) and OVXG5 (n = 3) using a linear mixed model. Results are fold changes (FC) of the metabolite abundance between the OVX and control groups, between the OVXG5 and the OVX groups and between the OVXG2 and the OVXG5 groups from month one to month five, among identified compounds by LC-MS
FC with a value > 1 indicates a relatively higher intensity present in treated ewes, whereas a value < 1 indicates a relatively lower intensity compared with their respective control animals
All FC values in bold are significant (p < 0.05)
p calculated using post-hoc Fisher’s least significant difference (LSD) test for pair-wise multiple comparison of the group means

ISSN: 1471-2474