The human arthritic hip joint is a source of mesenchymal stromal cells (MSCs) with extensive multipotent differentiation potential

Table 2 Primer details for semiquantitative RT-PCR

Gene	Primer sequences (5′-3′)	Annealing temperature (°c)	Product size (base pairs)	Cycles	MgCl₂
Housekeeping gene for internal control
EEF1α	Sense: AGGTGATTATCCTGAACCATCC Antisense: AAAGGTGGATAGTCTGAGAAGC	54.0	234	21	1 x
Adipogenic marker genes
LPL	Sense: GAGATTTCTCTGTATGGCACC Antisense: CTGCAAATGAGACACTTTCTC	51.0	239	30	1x
PPARγ	Sense: GCTGTTATGGGTGAAACTCTG Antisense: ATAAGGTGGAGATGCAGGCTC	61.0	297	35	1x
Osteogenic marker genes
Col I	Sense: GGACACAATGGATTGCAAGG Antisense: TAACCACTGCTCCACTCTGG	55.0	461	22	2x
Col X	Sense: CCCTTTTTGCTGCTAGTATCC Antisense: CTGTTGTCCAGGTTTTCCTGGCAC	54.0	468	40	1x
ALP	Sense: TGGAGCTTCAGAAGCTCAACACCA Antisense: ATCTCGTTGTCTGAGTACCAGTCC	51.0	454	33	1x
OC	Sense: ATGAGAGCCCTCACACTCCTC Antisense: GCCGTAGAAGCGCCGATAGGC	62.0	293	35	2x
Chondrogenic marker genes
AGG	Sense: GCCTTGAGCAGTTCACCTTCAntisense: CTCTTCTACGGGGACAGCAG	54.0	400	35	1x
Col II	Sense: TTTCCCAGGTCAAGATGGTC Antisense: CTTCAGCACCTGTCCACCA	51.0	155	31	1x
Sox-9	Sense: ATCTGAAGAAGGAGAGCGAG Antisense: TCAGAAGTCTCCAGAGCTTG	60.0	263	31	1x

EEF 1α Elongation factor 1α, LPL Lipoproteinlipase, PPARγ Peroxisome proliferator-activated receptor γ, Col I Collagen type I, Col X Collagen type X, ALP Alkaline phosphatase, OC Osteocalcine, AGG Aggrecane, Col II Collagen type II, Sox-9 Sex-determining region Y-box 9

ISSN: 1471-2474