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Fig. 5 | BMC Musculoskeletal Disorders

Fig. 5

From: The human arthritic hip joint is a source of mesenchymal stromal cells (MSCs) with extensive multipotent differentiation potential

Fig. 5

Histological and immunohistochemical analysis of chondrogenesis in cell-pellets after 27 d of pellet culture in presence of transforming growth factor-ß1. For inducing chondrogenesis pellets containing BM-MSCs and MSC-like cells from hyaline cartilage, LCF and the joint capsule were incubated with chondrogenic differentiation medium for 27 days. Contols were maintained in cell culture medium. After 27 days alcian blue staining (a) was performed for the detection of proteoglycans. While the controls remained negative all differentiated pellets showed positive alcian blue stainings. Immunohistochemical stainings of collagen type II (Col II; b) and collagen type X (Col X; right panel) were performed on pellet sections containing mesenchymal progenitor cells from bone marrow, hyaline cartilage (b), the LCF (c) and the joint capsule (d) after incubation in chondrogenic differentiation medium for 27 days. Controls were maintained in cell culture medium for the same duration of time (left panel). Positive staining for collagen type II (Col II; b) appeared red. Col II was detected in all differentiated pellet sections except from LCF (b, LCF), while moderate collagen type X (Col X; c) stainings were shown in all differntiated pellet sections and their controls after 27 days (c). Representative samples were captured at low (50x; black bar = 300 μm) and high (200x; black bar = 300 μm) magnification. Col II, collagen type I; Col X, collagen type X; d, days; diff., differentiation; LCF, ligamentum capitits femoris

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