Fig. 2From: The human arthritic hip joint is a source of mesenchymal stromal cells (MSCs) with extensive multipotent differentiation potentialAssay of adipogenesis in mesenchymal progenitor cells after 21 days in monolayer cell culture. For inducing adipogenesis monolayer cultures containing mesenchymal progenitor cells from bone marrow, hyaline cartilage, LCF and the joint capsule were incubated with adipogenic differentiation medium for 21 days. Controls were maintained in cell culture medium. Both native, unstained tissue samples (a) and Oil RedO stainings (b) from control and differentiatied samples were compared. In contrast to controls the formation of lipid droplets increased during day 12 to day 21 of adipogenesis (a) and all differentiated cultures stained positive for Oil Red O at the end of the differentiation period (b). Representative samples were captured at low (100x; black bar = 200 μm) and high (200x; black bar = 150 μm) magnification. d, days; diff., differentiation; LCF, ligamentum capitits femorisBack to article page