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Table 1 Primer sequences for RT-qPCR

From: Proper mechanical stress promotes femoral head recovery from steroid-induced osteonecrosis in rats through the OPG/RANK/RANKL system

Primer

Sequence (5′-3′)

OPG

F: TTTGCCTGGGACCAAAGTGAATGCAGAGAG

R: AGAAATGATAGGGAATCAGGTTCAATCAGT

RANK

F: TGGACAACCCAGGAAACCTTTCCTCCAAAA

R: GCCAGCCGAGACTACGGCAAGTACCTGCGC

RANKL

F: GGCCA GGTGG TCTGC AGCATCGCTCTGTTC

R: TTTATAGAATCCTGAGACTCCATGAAAACG

GAPDH

F: CGGACCAATACGACCAA

R: AGCCACATCGCTCAGACACC

  1. RT-qPCR Reverse transcription quantitative polymerase chain reaction, OPG Osteoprotegerin, RANK Receptor activator of nuclear factor kappa B, RANKL RANK ligand, GAPDH Glyceraldehyde-3-phosphate dehydrogenase, F Forward, R Reverse
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BMC Musculoskeletal Disorders

ISSN: 1471-2474

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