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Fig. 6 | BMC Musculoskeletal Disorders

Fig. 6

From: Proper mechanical stress promotes femoral head recovery from steroid-induced osteonecrosis in rats through the OPG/RANK/RANKL system

Fig. 6

Silencing OPG reduces the mechanical stress-promoted osteogenesis. a-b, mRNA (a) and protein (b) levels of OPG/RANK/RANKL were determined by RT-qPCR and western blot analysis, respectively. c-e, osteogenesis ability of extracted ex vivo cells of femoral head was determined by ALP staining (c), alizarin red staining (d) and RUNX2 immunofluorescence staining (e). Data are expressed as mean ± SD; one-way or two-way ANOVA and Tukey’s multiple comparisons test were used for data analysis; *p < 0.05; Replicates = 3

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