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Fig. 5 | BMC Musculoskeletal Disorders

Fig. 5

From: Proper mechanical stress promotes femoral head recovery from steroid-induced osteonecrosis in rats through the OPG/RANK/RANKL system

Fig. 5

Proper mechanical stress promotes differentiation of osteoblasts. a-c, osteogenesis ability of ex vivo cells of femoral head was determined by ALP staining (a), alizarin red staining (b) and RUNX2 immunofluorescence staining (c); d-e, mRNA (d) and protein (e) levels of OPG/RANK/RANKL were determined by RT-qPCR and western blot analysis, respectively. Data are expressed as mean ± SD; two-way ANOVA and Tukey’s multiple comparisons test were used for data analysis; *p < 0.05; Replicates = 3

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BMC Musculoskeletal Disorders

ISSN: 1471-2474

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