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Fig. 4 | BMC Musculoskeletal Disorders

Fig. 4

From: Proper mechanical stress promotes femoral head recovery from steroid-induced osteonecrosis in rats through the OPG/RANK/RANKL system

Fig. 4

OPG silencing reverses the proper stress-promoted bone recovery from ONFH. a-b, mRNA (a) and protein (b) levels of OPG/RANK/RANKL were determined by RT-qPCR and western blot analysis, respectively; c-d, Micro CT diagnosis was performed to determine parameters of trabecular in the cancellous bone of the femoral head; e, HE staining was performed to determine the morphology changes in the femoral head; f, immunohistochemistry staining was performed to determine the expression of VEGF, Bcl-2 and Caspase-3 in femoral head tissues; g, TUNEL was performed to determine the apoptotic cells in femoral head tissues. Data are expressed as mean ± SD; one-way or two-way ANOVA and Tukey’s multiple comparisons test were used for data analysis; *p < 0.05; Replicates = 3, n = 5 in each group

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