Fig. 3

OPG is highly-expressed while RANKL is lowly-expressed under proper mechanical stress. a-b, relative mRNA (a) and protein (b) levels of OPG/RANK/RANKL in the femoral head tissues were measured using RT-qPCR and western blot analysis. Data are expressed as mean ± SD; two-way ANOVA and Tukey’s multiple comparisons test was used to determine statistical significance; *p < 0.05; Replicates = 3, n = 5 in each group