Fig. 5From: LncRNA MCM3AP-AS1 regulates miR-142-3p/HMGB1 to promote LPS-induced chondrocyte apoptosisMCM3AP-AS1 over-expression promoted LPS-included chondrocyte apoptosis through HMGB1 and miR-142-3p. Chondrocytes were cultivated in medium containing 0, 500, 1000 and 2000 ng/ml LPS for 24 h under the aforementioned methods, followed by the measurement of MCM3AP-AS1, miR-142-3p and HMGB1 mRNA expression levels by qPCR (a). Cell apoptosis assay was performed to analyze the effects of MCM3AP-AS1, miR-142-3p and HMGB1 over-expression on the apoptosis of chondrocytes under 2000 ng/ml LPS treatment (b). Gene names on X-axis indicated the over-expression of this gene. c on X-axis, control cells, cells without transfection, NC on X-axis, negative control, cells transfected with empty vector or NC miRNA. Experiments were repeated 3 times and data were expressed as mean values. *, p < 0.05Back to article page