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Table 1 Primers for reverse-transcription PCR (RT-PCR) to determine osteogenic gene expression

From: Real-time detection of antibiotics cytotoxicity in rabbit periosteal cells using microfluidic devices with comparison to conventional culture assays

Gene

Primer sequence

GAPDH

Sense: 5′-GCCTGGTCACCAGGGCTGC-3′

Antisense: 5′-TGCTAAGCAGTTGGTGGTGCA-3′.

Cbfa1

Sense: 5′-CCGCACGACAACCGCACCAT-3′

Antisense: 5′-CGCTCCGGCCCACAAATCTC-3′

OPN

Sense: 5′-CCAAGTAAGTCCAACGAAAG-3′

Antisense: 5′-ATGTCTGCTCCTGTAGTGG-3’

OPNp

Sense: 5’-CAGAATGCTATGTCCTCAGA-3′

Antisense: 5′-CGTCCTCATCCTCATCAATA-3’

  1. GAPDH glyceraldehyde-3-phosphate dehydrogenase
  2. Cbfa1 core-binding factor subunit alpha-1
  3. OPN osteopontin
  4. OPNp ONP promoter