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Table 1 Primers for reverse-transcription PCR (RT-PCR) to determine osteogenic gene expression

From: Real-time detection of antibiotics cytotoxicity in rabbit periosteal cells using microfluidic devices with comparison to conventional culture assays

GenePrimer sequence
GAPDHSense: 5′-GCCTGGTCACCAGGGCTGC-3′
Antisense: 5′-TGCTAAGCAGTTGGTGGTGCA-3′.
Cbfa1Sense: 5′-CCGCACGACAACCGCACCAT-3′
Antisense: 5′-CGCTCCGGCCCACAAATCTC-3′
OPNSense: 5′-CCAAGTAAGTCCAACGAAAG-3′
Antisense: 5′-ATGTCTGCTCCTGTAGTGG-3’
OPNpSense: 5’-CAGAATGCTATGTCCTCAGA-3′
Antisense: 5′-CGTCCTCATCCTCATCAATA-3’
  1. GAPDH glyceraldehyde-3-phosphate dehydrogenase
  2. Cbfa1 core-binding factor subunit alpha-1
  3. OPN osteopontin
  4. OPNp ONP promoter
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