Fig. 2

Expression of DR3 on Cultured Post-menopausal Human CD14⁺ Monocyte Osteoclast Precursors. CD14⁺ monocytes were isolated from pre-menopausal (Pre) (n = 6) and post-menopausal females, without (PM) (n = 6) and with osteoporosis (OP) (n = 4). Cells were cultured on ivory discs for 7 days in media + MCSF. DR3 expression was determined by flow cytometry. a Gating strategy for detection of DR3. b Fold difference in CD14⁺ monocyte DR3 expression after 7 days and 12 days in culture. After 7 days pre-menopausal expression of DR3 was significantly elevated compared to the post-menopausal (p < 0.01) and post-menopausal with osteoporosis (p < 0.05) cultures. DR3 expression was not induced on CD14⁺ cells isolated from post-menopausal females after 12 days in culture. Statistical analysis performed by 1 way ANOVA with Dunnett’s multiple comparison test