Differential proteome analysis in adolescent idiopathic scoliosis patients with thoracolumbar/lumbar curvatures

Table 3 Identification of under- and overexpressed proteins by nanoLC-MS/MS

Spot No.	protein	NW^a	PI^b	Ratio^c	p-value^d	Score^e	Peptide^f	Coverage^g
U-1	Actin, cytoplasmic 1	42,052	5.29	4.98	0.00044	338	7	35%
U-2	Fibronectin	266,052	5.46	4.55	0.011	1640	25	22%
U-3	Vitamin D-binding protein	54,526	5.40	2.40	0.000033	8417	26	72%
U-4	Coagulation factor XIII A chain	83,728	5.75	2.33	0.00013	728	14	29%
U-5	Fibrinogen alpha chain	95,656	5.70	2.08	0.00059	17,627	26	44%
U-6	Complement factor H-related protein 1	38,766	7.38	2.06	0.00059	725	6	18%
U-7	Fibrinogen alpha chain	95,656	5.70	2.04	0.0005	10,673	25	42%
D-1	Complement C3	188,569	6.02	−3.19	0.0043	2819	16	21%
D-2	Complement C3	188,569	6.02	−2.84	0.0076	437	7	13%
D-3	Adiponectin	26,511	5.42	−2.50	0.021	59	1	0.17%
D-4	Prothrombin	42,108	5.31	−2.06	0.022	92	4	5%

a; Relative molecular mass, b; Isoelectric point, c; Mean volume ratio of AIS group vs. control group (AIS/Control, −X = 1/X), d; Student’s t-test, e; Based on the MOWSE logarithmic scoring algorithm; a score of ≥ 64 indicates significance, f; Number of peptide fragments matching the candidate protein, g; Percentage of sequences hit for registered full sequence

ISSN: 1471-2474