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Table 2 Primers for real-time polymerase chain reaction (PCR)

From: Expression of the RANK/RANKL/OPG system in the human intervertebral disc: implication for the pathogenesis of intervertebral disc degeneration

Genes

Assay IDa

Size (bp)

RANK

Hs00921372_m1

75

RANKL

Hs00243522_m1

67

OPG

Hs00900358_m1

74

IL-1β

Hs01555410_m1

91

MMP-3

Hs00968305_m1

78

MMP-13

Hs00233992_m1

75

18S rRNA

Hs99999901_s1

187

  1. RANK receptor activator of nuclear factor kappa B, RANKL rank ligand, OPG osteoprotegerin, IL interleukin, MMP matrix metalloproteinase, rRNA ribosomal RNA. aTaqMan Gene Expression Assays (Applied Biosystems)