Fig. 1From: Expression of the RANK/RANKL/OPG system in the human intervertebral disc: implication for the pathogenesis of intervertebral disc degenerationImmunohistochemical staining for receptor activator of nuclear factor kappa B (RANK)/RANK ligand (RANKL)/osteoprotegerin (OPG) in cultured annulus fibrosus (AF) and nucleus pulposus (NP) cells. All the cells were cultured in monolayer for 4 days: a-d AF; e-h NP. d, h Isotype controls. Samples were imaged using confocal microscopy. Immunoreactivity (green) is clearly seen in the AF, NP cells. Nuclei are stained with propidium iodide (red). Scale bar 10 μmBack to article page