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Fig. 1 | BMC Musculoskeletal Disorders

Fig. 1

From: Expression of the RANK/RANKL/OPG system in the human intervertebral disc: implication for the pathogenesis of intervertebral disc degeneration

Fig. 1

Immunohistochemical staining for receptor activator of nuclear factor kappa B (RANK)/RANK ligand (RANKL)/osteoprotegerin (OPG) in cultured annulus fibrosus (AF) and nucleus pulposus (NP) cells. All the cells were cultured in monolayer for 4 days: a-d AF; e-h NP. d, h Isotype controls. Samples were imaged using confocal microscopy. Immunoreactivity (green) is clearly seen in the AF, NP cells. Nuclei are stained with propidium iodide (red). Scale bar 10 μm

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