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Fig. 3 | BMC Musculoskeletal Disorders

Fig. 3

From: Transforming growth factor-β stimulates nerve growth factor production in osteoarthritic synovium

Fig. 3

Effect of TGF-β and an ALK5 inhibitor on the phosphorylation of p38 MAPK, NGF mRNA expression, and NGF protein production. Western blotting for phosphorylated p38 MAPK (p-p38) (a-b). Synovial cells were exposed to α-MEM (control), 10 ng/mL human recombinant (rh) TGF-β (TGF-β), or 10 ng/mL rhTGF-β + 5 μM SB505124 (TGF-β + SB505124) for 30 min before protein extraction and western blotting. Western blot for p38 and phosphorylated p38 MAPK (p-p38) (a). Bands were analyzed by densitometry using the NIH ImageJ software and normalized to p38 expression. Relative p-p38/p-38 expression was determined based on the expression in control samples (b). RT-PCR (c) and ELISA (d) for NGF. Synovial cells were exposed to α-MEM (control), 10 ng/mL rhTGF-β (TGF-β), or 10 ng/mL rhTGF-β + 5 μM SB505124 (TGF-β + SB505124) for 6 and 24 h before RT-PCR (c) or ELISA (d). Relative expression was determined based on the expression in control samples. Data indicate mean ± SE (n = 8). *p < 0.05 compared to control. Data indicate mean ± SE (n = 8). *p < 0.05 compared to control

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