Fig. 6

Effects of SI-613 on HAS1, HAS2, HAS3, HYAL1, HYAL2 and HYAL3 mRNA expression levels. HFLS-OA cells from three donors were cultured in IL-1β with PBS, 0.1% HA, or 0.1% SI-613 for 48 h. Cultures from individual patients were maintained as separate cultures and cells were not pooled. The mRNA expression levels of HAS1, HAS2, HAS3 (a), HYAL1, HYAL2 and HYAL3 (b) were measured by RT-qPCR. Values are represented as the means ± S.E. of three donors and shown as -fold increases in mRNA expression relative to control cells. Parametric Dunnett’s test was used for statistical analyses. *P < 0.05, **P < 0.01