Fig. 4
Effects of SI-613 and its chemical components on the molecular weight distribution of newly produced HA. HFLS-RA cells were cultured in [3H] glucosamine and IL-1β with the test materials for 48 h. Radiolabeled HA in the culture supernatant was separated by size exclusion HPLC using an OH pak SB-805 HQ column. a, The test materials were PBS (control), 0.01% HA, 0.01% SI-613, 1.4 μg/mL DF-Na or a mixture of 1.4 μg/mL DF-Na and 0.01% HA (DF-Na + HA). b, The test materials were 14, 1.4, 0.14 or 0.014 μg/mL DF-Na. The data represent results from a single HPLC run with a single well