Fig. 3
Time course of [3H] glucosamine incorporation into high molecular weight products. The HFLS-RA cells were cultured in [3H] glucosamine and IL-1β in the absence (a) or presence of 0.1% HA (b) and 0.1% SI-613 (c) for 8, 24, 36, 48 or 72 h. Radiolabeled HA in the culture supernatant was separated by size exclusion HPLC using an OH pak SB-805 HQ column. The data represent results from a single HPLC run with a single well