Fig. 1
Concentration-dependent effects of HA and SI-613 on the incorporation of [3H] glucosamine into high molecular weight products. HFLS-RA cells were cultured in [3H] glucosamine and IL-1β in the absence (solid squares) or presence of HA (open triangles) and SI-613 (open circles) for 48 h. The concentrations of HA and SI-613 were set at 0.1% (a), 0.01% (b) or 0.001% (c). Radiolabeled HA in the culture supernatant was separated by size exclusion HPLC using an OH pak SB-805 HQ column. The data represent results from a single HPLC run with a single well