Table 1 Sequences of the primers used in this study
From: Elevated leptin levels induce inflammation through IL-6 in skeletal muscle of aged female rats
Gene | Direction | Primer Sequence (5′–3′) | Product Size (bp) |
|---|---|---|---|
Il6 | F | CCAGTTGCCTTCTTGGGACT | 224 |
R | TCTGACAGTGCATCATCGCT | ||
Il1b | F | CCTCGTCCTAAGTCACTCGC | 156 |
R | GCAGAGTCTTTTTGACCCTCCT | ||
Tnfa | F | CTCTTCTCATTCCCGCTCGT | 104 |
R | GGGAGCCCATTTGGGAACTT | ||
MuRF1 | F | TGCAAGGAACACGAAGACGA | 170 |
R | ACAAGGAGCAAGTAGGCACC | ||
Atrogin1 | F | GGTCTCACGATCACCGACCT | 136 |
R | TCCACAGTAGCCGGTCTTCA | ||
Gapdh | F | TGC CAC TCA GAA GAC TGT GG | 129 |
R | TTCAGCTCTGGGATGACCTT |