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Fig. 8 | BMC Musculoskeletal Disorders

Fig. 8

From: Continuous low-intensity ultrasound attenuates IL-6 and TNFα-induced catabolic effects and repairs chondral fissures in bovine osteochondral explants

Fig. 8

Migration of chondrocytes under cLIUS and cytokines as measured in scratch assays. a The figure depicts phase contrast micrographs of the scratch area of chondrocytes at 12, 48 and 72 h (n = 3 scratch areas in 3 separate wells, 5× magnification; scale bar = 100 μm) in the presence or absence of IL-6 or TNFα under cLIUS at 14 kPa (5 MHz, 2.5 Vpp) for 20 min. Non-cLIUS-stimulated chondrocytes served as control (n = 3 scratch areas in 3 separate wells). Percentage scratch area closed by migrating chondrocytes at 6, 12, 24, 48 and 72 h normalized to the wound area at 0 h under no cytokine (b), IL-6 (c) and TNFα (d) were quantified by ImageJ software. Data represented as mean ± 95% confidence interval

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BMC Musculoskeletal Disorders

ISSN: 1471-2474

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